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Team:HokkaidoU Japan/Notebook/aggregation Week 13
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(26th characterization of aggregation) |
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| + | ==September 26th== | ||
| + | <div> | ||
| + | ==Characterization of Aggregation== | ||
| + | Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT construct. | ||
| + | #Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap. | ||
| + | #Suspended colonies into the medium. | ||
| + | #Incubated at 37C for 12 hrs at 180 rpm. For this incubation, pipetted 150 ul of this cultivating medium to 96-well microtiter plate every 2 hrs. | ||
| + | #Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager. | ||
| + | Result | ||
| + | </div> | ||
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Revision as of 20:31, 26 September 2012
September 26th
Characterization of Aggregation
Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT construct.
- Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
- Suspended colonies into the medium.
- Incubated at 37C for 12 hrs at 180 rpm. For this incubation, pipetted 150 ul of this cultivating medium to 96-well microtiter plate every 2 hrs.
- Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.
Result
