Team:Goettingen/week6-1

From 2012.igem.org

Revision as of 17:01, 25 September 2012 by B.gene (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Deutsch  / English 

#1 Selection / Swimming - 6th Week

Back to overview

V06_04


V06_04_1: preparation of swimming cultures and LB-swimming plates to perform swimming assay
  • Experiment:
    LB-Agar plates, composed of concentrations of 0.5% or 0.3% in addition to the standard LB-components were produced. Afterwards, 1 µL of DH10B-strains with P1-18C and P1-18M constructs were dropped onto plates.


V06_05


V06_05_1: Different promotor strengths were tested on different agar compositions.
  • Experiment:
    1 µL, 2 µL, and 5 µL of transformed strains (plasmids used: P1-18C-K3, P1-20E-K3, P1-18M-K2, P1-18M-K1, P1-18C-K2, P1-20E-K2) were tested on two different swimming agars, each. The agar was composed of: 1) 0.3% agar, 0.5% glucose, 1% yeast extract; 2) 0.3% agar, 0.5% glucose, 0,33% beef extract.
  • Observations & Results:
    The best swimming/growing conditions that could be identified were agar 1), no swimming at agar 2) and 5 µL of dropped baceria solution. The plates with 1 µL and 2 µL did not show any swimming/growth.


Back to overview

↑ Back to top!