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Team:Bielefeld-Germany/Results/Summary
From 2012.igem.org
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== TTHL - Laccase from ''Thermus thermophilus HB27'' == | == TTHL - Laccase from ''Thermus thermophilus HB27'' == | ||
| - | [[File:Bielefeld2012_TTHL6LFermentation.jpg|600px|thumb|left|Fermentation of ''E. coli'' KRX with BBa_K863012 (TTHL) in Bioengineering NFL22, scale: 6 L, autoinduction medium + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation increased when pO<sub>2</sub> was below 30 %, OD<sub>600</sub> taken every hour.]] | + | [[File:Bielefeld2012_TTHL6LFermentation.jpg|600px|thumb|left|Fermentation of ''E. coli'' KRX with BBa_K863012 (TTHL) in Bioengineering NFL22, scale: 6 L, [https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation increased when pO<sub>2</sub> was below 30 %, OD<sub>600</sub> taken every hour.]] |
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Revision as of 10:51, 23 September 2012
Summary
BPUL - Laccase from Bacillus pumilus DSM 27 (ATCC7061)
After the measurement of activity of BPUL we made a scale-up and fermented E. coli KRX with <partinfo>BBa_K863000</partinfo> in Braun Biostat B with a total volume 3 L. Agitation speed, pO2 and OD600 were determined and illustrated in the following graph. We got a long lag phase,
ECOL - Laccase from Escherichia coli BL21 (DE3)
XCCL - Laccase from Xanthomonas campestris pv. campestris B100
BHAL - Laccase from Bacillus halodurans C-125
TTHL - Laccase from Thermus thermophilus HB27
PCIL - Laccase from Pycnoporus cinnabarinus
TVEL5 - Laccase from Trametes versicolor
TVEL10 - Laccase from Trametes versicolor
TVEL13 - Laccase from Trametes versicolor
TVEL20 - Laccase from Trametes versicolor
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