Team:Bielefeld-Germany/Public relations Overview

From 2012.igem.org

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                Human Practice
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<a href="#1"><strong>Overview</strong>
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<strong>Molecular</strong>
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Genetic engineering protocols
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Upstreaming and downstreaming
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Analytical methods
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<a href="#7"><strong>Environment</strong>  
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Enzymes, kits, chemicals, buffers
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Genetics">Molecular</a></h3>
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<h3>Overview</h3>
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An important part of iGEM is Human Practice, which we understand not only as communication with a broader public but also as reflection of the project in a general aspect. This includes biosafety and biosecurity, social aspects as well as the implementation of the project into industrial processes and evaluation of experts and environmental issues. Therefore economical factors and political terms have to be considered. Finally we want to show the potential of the project as a possible realistic alternative to conventional and traditional methods of waste water treatment concerning the depletion of steroid hormones as estrogen and other pharmaceutical relevant substances.
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In the sections expert, politics and industry we tried to evaluate our project in economical manner with the consideration of political terms.
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The sections environment, education and outreach focus on social aspects, including the environmental situation and communication with a broader public.
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In this section of our protocol pages you can read more about our methods for cloning and BioBrick assembly.
 
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Genetic engineering is a basic tool of synthetic biology. With the help of standardized DNA building blocks (BioBricks) it is fairly easy to create new and modify existing natural systems. The methods we have used in our project to create BioBricks and to modify, mutate, transform and analyse DNA are presented in this section. Methods used: Electroporation; chemical transformation; Standard, Freiburg, Gibson and 3A BioBrick assembly; restriction analysis; colony PCR; site directed mutagenesis. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Genetics">read more</a>
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<img src="https://static.igem.org/mediawiki/2012/6/66/Bielefeld2012_Expert1.jpg" />
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<h3>Expert</h3>
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In this section you can read more about the expert classification we got for our project. We presented our project to get feedback for our ideas.
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We attended at the strategy process "Biotechnologie2020+".
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<p> <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/StrategicProcess">read more</a></p>
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<br>
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The CAS conference for Synthetic Biology gave us the opportunity to present a poster about our project.
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<p> <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/CAS_conference">read more</a> </p>
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Downstream-processing">Production</a></h3>
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<h3>Politics</h3>
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These are the protocols for the cultivations and the downstream processing.
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Here you can find our project evaluation with Rene R&ouml;spel, member of the german parliament, the ''Bundestag''.  
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Before one is able to work with a cell-free system based on biological material, the needed proteins have to be produced and purified first. These methods and the ones we used to characterize BioBricks in vivo are presented in this section. Used methods: Cultivations in shaking flasks and bioreactor; protein clean-up from medium, periplasm, whole cell and inclusion bodies; UF / DF; IEX; Ni-NTA columns and chromatography; recrystallization and immobilization of S-layer proteins. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Downstream-processing">read more</a>
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For the development of a project from planning to application political terms are important. Not only the economical factors but also the public opinion and acceptance depend on the political situation. Furthermore bioethics are especially important when developing a project in modern life sciences. Therefore we discussed about bioethics as well as regulations.
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<p><a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/Politics">read more</a></p>
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Protocols for the analytical methods we used.  
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An important part of a project development is the evaluation in cooperation with potential partners. Therefore we visited two sewage treatment plants and together with the plant managers we could work out an easy way for a future application. For our potential application when plan to use existing structures to present a real alternative to commonly used sewage treatment.  
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DNA and proteins are very small and cannot be seen by the naked eye. To control the success and the results of your upstream and downstream processes, analytical methods are necessary that give reliable results to make DNA or proteins in any way visible for you. The analytical methods we used in our project can be found in this section. Used methods: Fluorescence measurement; SDS-PAGE; MALDI-TOF; HPLC; LC-ESI-qTOF-MS/MS; molecular beacons; extraction. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Analytics">read more</a>
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<a href="https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant">read more</a>
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<h3>Outreach</h3>
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In Germany people are very suspicious when it comes to Synthetic Biology. Despite of strict regulations and decisive risk and technological impact assessment the public opinon is marked by prejudices and anxiety for biotechnology. Therefore outreach is a central part of every German iGEM project.
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</p>
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<p>
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To inform the public the German iGEM teams arranged a SynBioDay, that was held all over Germany. We presented posters, organized experiments and as a special additional we invited the iGEM team from SDU-Denmark to help us. <p><a href="https://2012.igem.org/Team:Bielefeld-Germany/StreetScience">read  more</a></p>
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We did a waffle sell and a poster presentation in our university. <p> <a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/PosterPresentations">read  more</a></p>
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<h3>Education</h3>
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Synthetic Biology is new and unknown life sciences. Therefore we hosted a student academy for interested pupils to learn about Synthetic Biology. The student academy was addressed to talented pupils of higher grades in order to deepen their understanding of molecular processes in cells. Together with the pupils we worked on practical experiments, while telling them about SynBio. Of course we also took the opportunity to present our project to a young audience and had an interesting discussion.
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<a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/StudentAcademy">read more</a>
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<img src="https://static.igem.org/mediawiki/2012/4/44/Bielefeld2012_Environment.jpg"  />
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<h3>Environment</h3>
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We wanted to evaluate the urgency of the current situation in marine ecological system. Therefore we had an interview with Nicolai Fricke, an expert of the <a href="http://www.vti.bund.de/de/startseite/institute/foe.html">Heinrich von Thünen-Institute of Fisheries Ecology.</a> 
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<a href="https://2012.igem.org/Team:Bielefeld-Germany/Environment">read more</a>
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<h3><a style="text-decoration:none; color:black;" href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Materials">Material</a></h3>
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<h3>Press</h3>
<p class="more">
<p class="more">
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Chemicals, enzymes and kits we used in our lab work.  
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Here you can find the articles to our project. Feel free to take a look, because we tried hard to have an open communication with the public. That's why we published two press releases and fortunately triggered a huge media response: many many web pages took up our press release resulting in several articles in online magazines and articles in the printed press. Furthermore we talked in two radio broadcastings and are part of a TV documentation about SynBio.
</p>
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<p>
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Chemical and biological reactions need defined conditions to work as expected. The chemicals, enzymes, kits, buffers and media we used in our project are listed in this section. <a href="https://2011.igem.org/Team:Bielefeld-Germany/Protocols/Materials">read more</a>
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<a href="https://2012.igem.org/Team:Bielefeld-Germany/Human_Practices/Press">read more</a>
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Latest revision as of 03:37, 27 October 2012

Human Practice

Overview

An important part of iGEM is Human Practice, which we understand not only as communication with a broader public but also as reflection of the project in a general aspect. This includes biosafety and biosecurity, social aspects as well as the implementation of the project into industrial processes and evaluation of experts and environmental issues. Therefore economical factors and political terms have to be considered. Finally we want to show the potential of the project as a possible realistic alternative to conventional and traditional methods of waste water treatment concerning the depletion of steroid hormones as estrogen and other pharmaceutical relevant substances. In the sections expert, politics and industry we tried to evaluate our project in economical manner with the consideration of political terms. The sections environment, education and outreach focus on social aspects, including the environmental situation and communication with a broader public.

Expert

In this section you can read more about the expert classification we got for our project. We presented our project to get feedback for our ideas.

We attended at the strategy process "Biotechnologie2020+".

read more


The CAS conference for Synthetic Biology gave us the opportunity to present a poster about our project.

read more

Politics

Here you can find our project evaluation with Rene Röspel, member of the german parliament, the ''Bundestag''.

For the development of a project from planning to application political terms are important. Not only the economical factors but also the public opinion and acceptance depend on the political situation. Furthermore bioethics are especially important when developing a project in modern life sciences. Therefore we discussed about bioethics as well as regulations.

read more

Industry

An important part of a project development is the evaluation in cooperation with potential partners. Therefore we visited two sewage treatment plants and together with the plant managers we could work out an easy way for a future application. For our potential application when plan to use existing structures to present a real alternative to commonly used sewage treatment.

read more

Outreach

In Germany people are very suspicious when it comes to Synthetic Biology. Despite of strict regulations and decisive risk and technological impact assessment the public opinon is marked by prejudices and anxiety for biotechnology. Therefore outreach is a central part of every German iGEM project.

To inform the public the German iGEM teams arranged a SynBioDay, that was held all over Germany. We presented posters, organized experiments and as a special additional we invited the iGEM team from SDU-Denmark to help us.

read more

We did a waffle sell and a poster presentation in our university.

read more

Education

Synthetic Biology is new and unknown life sciences. Therefore we hosted a student academy for interested pupils to learn about Synthetic Biology. The student academy was addressed to talented pupils of higher grades in order to deepen their understanding of molecular processes in cells. Together with the pupils we worked on practical experiments, while telling them about SynBio. Of course we also took the opportunity to present our project to a young audience and had an interesting discussion.

read more

Environment

We wanted to evaluate the urgency of the current situation in marine ecological system. Therefore we had an interview with Nicolai Fricke, an expert of the Heinrich von Thünen-Institute of Fisheries Ecology.

read more

Press

Here you can find the articles to our project. Feel free to take a look, because we tried hard to have an open communication with the public. That's why we published two press releases and fortunately triggered a huge media response: many many web pages took up our press release resulting in several articles in online magazines and articles in the printed press. Furthermore we talked in two radio broadcastings and are part of a TV documentation about SynBio.

read more


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Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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