Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL).
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]
 +
:We successfully produced purified and identified active laccases of ''E. coli'' BL21(CueO named ECOL), ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL), ''Bacillus halodurans'' C-125 laccase (Lbh1 named BHAL) and ''Thermus thermophilus'' HB27 (tthL named TTHL).
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results ECOL]  
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results ECOL]  
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results BPUL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results BPUL]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results TTHL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results TTHL]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results BHAL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results BHAL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.   
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]
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:We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.   
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced the laccase TVEL5 from ''Trametes versicolor'' in ''P. pastoris'' and showed the activity of the produced enzymes.
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]  
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   
 +
:We successfully produced the laccase TVEL5 from ''Trametes versicolor'' in ''P. pastoris'' and showed the activity of the produced enzymes.
 +
:[https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel5 Results]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results ECOL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]
 +
:Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results BPUL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results ECOL]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results TTHL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results BPUL]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results BHAL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results TTHL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results BHAL]
   
   
   
   
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   
 +
:We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor'' laccase (named TVEL0), ''E. coli'' BL21 laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL) and measured their activity.  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  
 +
:We successfully immobilized ''Trametes versicolor'' laccase (named TVEL0), ''E. coli'' BL21 laccase (CueO named ECOL) and ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL) and measured their activity.  
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results TVEL0]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results ECOL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results ECOL]
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results BPUL]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results BPUL]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function.  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  
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:We successfully improved a BioBrick by clarifying its true function.  
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[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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:[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created our own database to organize our samples.
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:[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created our own database to organize our samples.
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[https://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created a model which simulates the oxidation of ABTS.
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:[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created a model which simulates the oxidation of ABTS.
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[https://2012.igem.org/Team:Bielefeld-Germany/Modell Results]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Modell Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We conducted a feasibility study of our project with sewage plant experts.
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  
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:We conducted a feasibility study of our project with sewage plant experts.
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[https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant Results]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We did an outstanding outreach work.
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  
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:We did an outstanding outreach work.
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[https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview Results]
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:[https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview Results]
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[[File:Seal_gold.png‎|30px|frameless|left]] We won a gold medal.
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[[File:Bielefeld-Germany2012_Goldmedaillie.png‎|30px|frameless|left]]  
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:We won a gold medal.
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[[File:Bielefeld-Germany2012_Qualified.png‎|30px|frameless|left]]
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:We qualified for the Boston finals
</b>
</b>

Revision as of 23:33, 26 October 2012


Project Achievements

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified active laccases of E. coli BL21(CueO named ECOL), Bacillus pumilus DSM 27 laccase (CotA named BPUL), Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and Thermus thermophilus HB27 (tthL named TTHL).
Results ECOL
Results BPUL
Results TTHL
Results BHAL


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. We were able to show that the vector is functioning as expected.
Results
Bielefeld2012 haken grau.jpg
We successfully produced the laccase TVEL5 from Trametes versicolor in P. pastoris and showed the activity of the produced enzymes.
Results
Bielefeld2012 haken grau.jpg
Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
Results ECOL
Results BPUL
Results TTHL
Results BHAL


Bielefeld2012 haken grau.jpg
We established a method for the degradation of several compounds with the help of our self-produced laccases. The HPLC analysis showed that our produced bacterial laccases are able to degrade estradiol with and without addition of ABTS. BPUL is able to degrade ethinyl estradiol with addition of ABTS. LC-MS measurements showed degradation products which could not be identified but were further analyzed using MS/MS. The resulting products have been discussed with an organic chemist.
Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Trametes versicolor laccase (named TVEL0), E. coli BL21 laccase (CueO named ECOL) and Bacillus pumilus DSM 27 laccase (CotA named BPUL) and measured their activity.
Results TVEL0
Results ECOL
Results BPUL


Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function.
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]


Bielefeld2012 haken grau.jpg
We successfully created our own database to organize our samples.
Results


Bielefeld2012 haken grau.jpg
We successfully created a model which simulates the oxidation of ABTS.
Results


Bielefeld2012 haken grau.jpg
We conducted a feasibility study of our project with sewage plant experts.
Results


Bielefeld2012 haken grau.jpg
We did an outstanding outreach work.
Results


Bielefeld-Germany2012 Goldmedaillie.png
We won a gold medal.
We qualified for the Boston finals


55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg