Team:Bielefeld-Germany/Labjournal/week4

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Contents

Labjournal

Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19 Week20 Week21


Week 4 (05/21 - 05/27/12)

weekly seminar:

  • Lab service: Isabel
  • We try to establish a collaboration with the iGEM team from SDU-Denmark
  • Got our distribution kits
  • First successful cloning and cultivations
  • Who wants to be a summer school teacher?
  • We will not travel to the ACHEMA because only local teams are invited
  • Do we want to participate in the Biolympics? (It's a sports party with fun organized by the [http://bts-ev.de/ bts])


Monday May 21st

  • Team Modeling: Our aims for modeling:
    • model the expression of the laccases in the organisms.
    • model the activity of our enzymes.
    • model the interesting parts of a clarification plant (the part witch are interesting for our cleaner.
  • Team Bacterial Laccases: We wanted to clone our laccase PCR products from Xanthomonas campestris and E. coli in pSB1C3 backbone. Therefore we did some restriction digests on the PCR products and the vector BBa_J04450.

Tuesday May 22nd

Team Bacterial Laccases: We picked colonies from transformed BBa_J04450 and plated them on new LB plates with chloramphenicol.

Wednesday May 23rd

  • Team Student Academy: Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see here. Got intense red fluorescing colonies but no green fluorescing colonies. Made a backup of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]. Asking for other plasmids containing GFP at the working groups of our University.
  • Team Bacterial Laccases:
    • After there were no colonies on our pSB1C3 + CopA (Xanthomonas campestris) plate we did the transformation with the same ligation preparation again. For the other ligation with pSB1C3 + Cueo (E. coli) we started colony PCRSs to find postive colonies. Sadly the colony PCR showed no product.
    • Purification of the T. thermo laccase PCR product out of agarose gel.

Thursday May 24th

  • Team Student Academy: Made a liquid culture of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] at 30°C. There was no fluorescence. Furthermore [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] was transformed. Also got intense red fluorescing colonies.

Friday May 25th

Team Bacterial Laccases:

  • We had to do the PCR on T. thermo laccase again, because before the amount of purified PCR product was very low.

Saturday May 26th

Sunday May 27th