Team:Bielefeld-Germany/Labjournal/week4

From 2012.igem.org

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===Wednesday May 23rd===
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*''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got red fluorescing colonies but no green fluorescing colonies.
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*''' Team Student Academy:''' Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see [https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2#Monday_May_7th here]. Got red fluorescing colonies but no green fluorescing colonies. Asking for other plasmids containing GFP at the working groups of our University.
===Thursday May 24th===
===Thursday May 24th===

Revision as of 14:38, 24 August 2012

Contents

Labjournal

Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18


Week 4 (05/21 - 05/27/12)

Monday May 21st

  • Team Modeling: Our aims for modeling:
    • model the expression of the laccases in the organisms.
    • model the aktivity of our enzyms.
    • model the interesing parts of a clarification plant (the part witch are interesing for our cleaner.

Tuesday May 22nd

  • Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.

Wednesday May 23rd

  • Team Student Academy: Repetition of the transformation of [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 BBa_I13522] with new competent cells. For setup see here. Got red fluorescing colonies but no green fluorescing colonies. Asking for other plasmids containing GFP at the working groups of our University.

Thursday May 24th

Friday May 25th

Saturday May 26th

Sunday May 27th