Team:Bielefeld-Germany/Labjournal/week21

From 2012.igem.org

(Difference between revisions)
(→Thursday September 20th)
(→Week 21 (09/17 - 09/23/12))
 
(87 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Bielefeld/Head}}
{{Team:Bielefeld/Head}}
-
<div style="text-align:justify;">
 
-
==Week 21 (09/17 - 09/23/12)==
+
<html>
 +
<style type="text/css">
-
===Monday September 17th===
+
ul {list-style-image:none;}
-
===Tuesday September 18th===
+
#bodyContent{
-
* '''Team Cellulose Binding Domain:'''
+
    background-color: white;
-
**Primer arrived:
+
}
-
**Gradient-PCR with the CBDcex(T7)+GFP_His-plasmid and S3N10-CBDcex_RV and S3N10-GFP_FW primer-mix. the right product appeared only at 66,5°C; Lower: wrong product; Higher: no product
+
-
**Following PCR at 66,5°C(50µL) Result: main product at 2 kbp
+
-
**Alternativly took the 20 µL-tube with right product and added 0,5 µL ''Dpn''I for over-night digestion.
+
-
**Gradient-PCR with CDBcex(T7)+GFP_His-Template and CBDcex_Freiburg+GFP_Freiburg-primer-mix.
+
-
**Gradient-PCR with CDBclos(T7)(9)+GFP_His-Template and CBDclos_Freiburg+GFP_Freiburg-primer-mix.
+
-
===Wednesday September 19th===
+
</style>
-
* '''Team Cellulose Binding Domain:'''
+
-
**Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.
+
-
**Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced) with the CBDcex_Freiburg and GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures from 57°C to 64°C; merged the correct fractions and cleaned them up through the gel.
+
-
**Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I.
+
-
**Ligated J61101 and CBDclos with GFP_Freiburg and transformed it into KRX.
+
-
===Thursday September 20th===
+
<!-- navigator -->
-
* '''Team Cellulose Binding Domain:'''
+
<div id="nav" class="tabs">
-
**No Colonies on the S3N10-Clos-transformations-dish with the blunt-end ligation; reason: forgotten to phosphorylate the PCR-product.
+
<div class="scroller">
-
**A few colonies on the dish with the CBDclos+GFP and the constitutive [http://partsregistry.org/Part:BBa_J61101 J23100 + J61101] promoter. But none of them is glowing.
+
<ul style="list-style-type:none">
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
 +
                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
 +
                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
 +
<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
-
===Friday September 21st===
+
</ul>
-
===Saturday September 22nd===
+
-
===Sunday September 23rd===
+
-
Sunday
+
 +
</div>
 +
</div>
 +
</html>
-
{{Team:Bielefeld/Sponsoren}}
+
<div style="text-align:justify;">
 +
 
 +
==Week 21 (09/17 - 09/23/12)==

Latest revision as of 21:32, 25 September 2012


==Week 21 (09/17 - 09/23/12)==
Retrieved from "http://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"