Team:Bielefeld-Germany/Labjournal/week21

From 2012.igem.org

(Difference between revisions)
(Wednesday September 19th)
(Wednesday September 19th)
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===Wednesday September 19th===
===Wednesday September 19th===
* '''Team Cellulose Binding Domain:'''Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.  
* '''Team Cellulose Binding Domain:'''Stopped over-night-''Dpn''I-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.  
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**Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced)with the CBDcex_Freiburg+GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures form 57°C to 64°C; merged the products and cleaned them through gel and clean-up kit.
+
**Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced) with the CBDcex_Freiburg and GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures from 57°C to 64°C; merged the correct fractions and cleaned them up through the gel.
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**Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I
+
**Digested the cleaned up product with ''Spe''I and ''Xba''I and ''Dpn''I.
===Thursday September 20th===
===Thursday September 20th===

Revision as of 16:10, 19 September 2012

Contents

Week 21 (09/17 - 09/23/12)

Monday September 17th

Tuesday September 18th

Wednesday September 19th

  • Team Cellulose Binding Domain:Stopped over-night-DpnI-digestion of S3N10-clos-GFP_his PCR-product and cleaned up in gel. Ligated the PCR-product with itself via Blunt-end-ligation and transformed it into KRX. Plated on Select-Agar.
    • Gradient-PCRs of the CDBcex(T7)+GFP-plasmid and a CBDclos(T7)+GFP-plasmid (unsequenced) with the CBDcex_Freiburg and GFP_Freiburg-compl and the CBDclos_Freiburg and GFP_Freiburg_compl primers, respectively. The CBDcex-setup showed no correct product, but the CBDclos-setup had the right bands at temperatures from 57°C to 64°C; merged the correct fractions and cleaned them up through the gel.
    • Digested the cleaned up product with SpeI and XbaI and DpnI.

Thursday September 20th

Friday September 21st

Saturday September 22nd

Sunday September 23rd

Sunday


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