Revision as of 18:56, 12 August 2012

Labjournal

Start of our WET LAB time.
Generating new competent E.coli KRX cells.
Cultiviation of Xanthomonas campestris B100 and E. coli BL21(DE3). The bacterial strains we got from a working group at our University. After cultivation we isolated the genomic DNA. The DNA was needed as template for PCRs to purify the wanted laccase ORFs.
Sending requests to working groups for different plasmids , which have already worked with laccases and described them in their papers. Unfortunately just one answer came back (thanks a lot to them).So we got a vector with the laccase-ORF CotA from Bacillus pumilus ATCC7061 from the Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials in Switzerland.

weekly seminar:

Do we want to order strains of Trametes versicolor and Trametes villosa?
Gathering information about signal sequences in yeast
Decision to create a database, so that we can easily number and inscribe our lab results
Decision to arrange a summer school for pupils in their last year before the final exams
Discussion about how to meet a member of the german Bundestag (the german parliament)

@@ Line 21: / Line 21: @@
 ==Week 1 (04/30 - 05/06/12)==
-===Monday April 30th===
+* Start of our WET LAB time.
-===Tuesday May 1st===
+* Generating new competent ''E.coli KRX cells''.
-===Wednesday May 2nd===
+* Cultiviation of ''Xanthomonas campestris B100'' and ''E. coli BL21(DE3)''. The bacterial strains we got from a working group at our University. After cultivation we isolated the genomic DNA. The DNA was needed as template for PCRs to purify the wanted laccase ORFs.
-===Thursday May 3rd===
+* Sending requests to working groups for different plasmids , which have already worked with laccases and described them in their papers. Unfortunately just one answer came back (thanks a lot to them).So we got a vector with the laccase-ORF CotA from ''Bacillus pumilus ATCC7061'' from the Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials in Switzerland.
-===Friday May 4th===
-===Saturday May 5th===
-===Sunday May 6th===
+weekly seminar:
+* Do we want to order strains of ''Trametes versicolor'' and ''Trametes villosa''?
+* Gathering information about signal sequences in yeast
+* Decision to create a database, so that we can easily number and inscribe our lab results
+* Decision to arrange a summer school for pupils in their last year before the final exams
+* Discussion about how to meet a member of the german ''Bundestag'' (the german parliament)