Team:Valencia/BG-11HGs

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BG-11 for Harvard/Golden in medium solid.

Reagents and Materials

• 100ml BG-11 Harvard/Golden liquid
• Antibiotics.
• 3% Agar.

Protocol

1. Add 100ml of BG-11 Harvard/Golden liquid in a Erlenmeyer flask.
2. Add 3g of agar in a flask wich contains BG-11.
3. Set pH at 7’4 for Synechococcus elongatus to come from Harvard. In the other hand, set the pH at 8’9 for S. elongatus to come from Golden.
4. Autoclave at 121ºC during 20 minutes while label and prepare the plates.
5. Add the antibiotics. Cool BG-11 with the agar.
6. Fill BG-11 in the plates and leave until solidify.