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V05_15
Preparing Media
- Experiment:
Preparation of LB medium and LB-Amp plates (recipe see here). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.
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V05_16
Isolation of pUC18 plasmid DNA
- Experiment:
Isolation of pUC18 from DH10B using peqGOLD Miniprep Kit (Peqlab).
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V05_18
Insertion of tar into pUC18
- Experiment:
Double digest of tar and pUC18 using EcoRI, PstI and Orange Buffer (ThermoScientific). Preparation of chemical competent DH10B cells for transformation.
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