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From 2012.igem.org

Contents 1 Labjournal 1.1 Week 10 (07/02 - 07/08/12) 1.1.1 Monday July 2nd 1.1.2 Tuesday July 3rd 1.1.3 Wednesday July 4th 1.1.4 Thursday July 5th 1.1.5 Friday July 6th 1.1.6 Saturday July 7th 1.1.7 Sunday July 8th

Labjournal

Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19

Week 10 (07/02 - 07/08/12)

Monday July 2nd

• Sending a request for plasmids containing cDNA sequences of five different laccases from Trametes versicolor and Pycnoporus cinnabarinus from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald.

Tuesday July 3rd

Wednesday July 4th

• Team Student Academy: Final meeting with all instructors of the student academy to talk about all details.

Thursday July 5th

• Team shuttle vector: Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primerpair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primerpair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primerpair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primerpair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r.

Friday July 6th

• Team Student Academy: The two presentations were prepared and the barbecue for the pupils was organized. In the lab the last preparations were made.

Saturday July 7th

Sunday July 8th