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Team:Bielefeld-Germany/Labjournal/week7
From 2012.igem.org
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 7 (06/11 - 06/17/12)
Monday June 11th
- Team Sequencing: Sequencing of the first finished biobricks.
Tuesday June 12th
Wednesday June 13th
- Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.
- Team Bacterial laccase: since the GC amount of the S. griseus and S. lavendulae laccases are high we used betain to solve the PCR problem.
-> Addition of betain did not changed anything from the result we still haven't had our laccase DNA.
Thursday June 14th
- Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase does not seem to be totally satisfied at 4°C. I would not either.
- Team Bacterial laccase: Because our PCRs have not worked well we thought it may depend on the primer annealing temperature so we did gradient PCR with the same conductions as before.
