Team:Paris Bettencourt/Suicide
From 2012.igem.org
Suicide Group
Contents |
Overview
Our goal is to engineer a synthetic toxin-antitoxin system from the wild type colicin E2. This synthetic toxin-antitoxin system is receptor specific, allows for population level cell death, destruction of the genetic material, and will function on a tunable delay.
Objectives
- Design an anti-toxin part from the colicin E2 immunity gene that confers immunity against the colicin E2 activity protein
- Develop RFP labeled MG1655 Z1 strains immune to colicin E2 activity protein
- Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of WT ColE2 cells
- Design a colicin E2 activity protein part under the control of a constitutive promoter and establish in immune MG1655 Z1 cells to generate synthetic ColE2 cells
- Measure viability of immune and vulnerable MG1655 Z1 cells in the presence of synthetic ColE2 cells
- Tune the concentrations of toxin (colicin E2 activity protein
- Develop RFP labeled MG1655 Z1 strains immune to colicin E2 activity protein) and anti-toxin (colicin E2 immunity protein)
- Combine our system with the Restriction Enzyme Group`s work, measure viability
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results