Team:University College London/Protocols/ColonyDNAExtraction

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PCR

Step 1 - Picking Colony: Using a pipette select an isolated colony Step 2 - Deposit: Dip the colony in 10ul of dH20, preferably in screw top container Step 3 - Boiling Stage:Boil the dH20 100 for 5-10mins Step 4 - Centrifuge: Time: 1 min RPM: 8000 Temp: 18oC Step 5 - Remove Supernatant: The supernatant contains the DNA template.