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Team:Bielefeld-Germany/Labjournal/week7
From 2012.igem.org
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 7 (06/11 - 06/17/12)
Monday June 11th
- Team Sequencing: Sequencing of the first finished biobricks.
Tuesday June 12th
- Team Site Directed Mutagenesis: Generated the first Site-Directed-Mutagenesis primer for Bacillus pumilus (two sites), Xanthomonas Campestris (two sites), Escherichia coli (one site)und Thermus thermophilus (one site).
- Team Cellulose Binding Domain: Searched NCBI for Cellulose, Chitin & Keratin binding motifs in accessable organisms; found two Chitin-binding-domains in Bacillus Halodurans
Wednesday June 13th
- Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.
Thursday June 14th
- Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase doesn´t seem to be totally satisfied at 4°C. I wouldn´t either.
