Team:BostonU/Project Overview

• Our Team
  • Team
  • Summer Fun
• Project
  • Project Overview and Abstract
  • Introduction to Characterization
  • Methodology Overview
  • Results Summary
• Achievements
  • Data Collected
  • Parts Submitted
  • MoClo Kit
  • Medal Fulfillment
• Notebook
  • Protocols
  • Clotho and Eugene
  • Weekly Notebook
• Considerations
  • Synthetic Biology Advancement
  • New England iGEM Regional Meeting
  • Human Practices
  • Safety
• Acknowledgements
  • Collaborations
  • Acknowledgements

Project Overview

Abstract

Our project aims to introduce a standardized protocol for the characterization of genetic circuits using flow cytometry. We built a vast number of both simple and complex genetic circuits that were characterized using flow cytometry. These genetic circuits were built using an assembly technique called MoClo (developed by Weber et al., 2011), which involves a multi-way, one-pot digestion-ligation reaction, enabling faster and more efficient construction of genetic circuits. We converted a large subset of BioBrick™ Parts from the Registry into MoClo Parts using PCR and cloning strategies. We built and characterized various genetic circuits using MoClo Parts and compared them against their pre-existing BioBrick™ counterparts in order to compare the characterization results from the two assembly techniques. We also created a standardized data sheet to be included in the Registry of Standard Biological Parts for each Part we characterized to easily share our data with the synthetic biology community.