Team:University College London/Module 6/Characterisation

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Module 6: Containment

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

Characterisation

In order to determine the activity of the exonuclease, we will be utilising DNAse agar. This agar contains DNA fragments that would be cleaved by the exonuclease, leaving the regions surrounding the colonies free of genetic material. The agar plates can then be stained with a DNA indicator such as Hydrochloric Acid or Toludine Blue, allowing the DNA voids in the agar to be visible.


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