Team:University College London/Protocols/Nanodrop

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== Nanodrop ==

Software ND-1000 Model:

1.Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software

2.Wipe and add elution buffer as negative control. Click blank in ND-1000 software

3.Wipe and add 1 µl sample

4.On the software set lambda to 260nm

5.Lower the lever arm and click measure in ND-1000 software

6.Take readings for concentration and purity

7.Once measurement complete, wipe surface