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Team:Bielefeld-Germany/Amsterdam/Results
From 2012.igem.org
Summary
coming soon
Laccases
The iGEM Team successfully produced again four active bacterial laccases:
All of the bacterial laccases were accomplished to purify. The purified laccases . Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol. The laccase of TVEL0 fromTrametes versicolor was produced and tested for activity on oaxidation of ABTS.
Substrate Analysis
After the regionals Team Activity Test, Team Immobilization and Team Substrate Analysis got laccases from the same pool from the Cultivation Team. With these laccases the degradation experiments of estradiol and ethinyl estradiol were repeated for BPUL and ECOL. The new laccases BHAL and TTHL were characterized for estradiol and ethinyl estradiol degradation, too.
For more information about the new resultsclick here.
Possible resulting degradation products were further analysed via LCMS-MS.
Cellulose binding domain
A lot of efforts were made, to change the order of the fusion proteins, to change the promoter and the RBS and to insert a different linker between the cellulose binding domain and the reporter GFP, but to the last day of lab work no green glowing colony to work with and execute the binding assay could be generated.
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