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Team:Bielefeld-Germany/Amsterdam/Results
From 2012.igem.org
Summary
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Laccases
The iGEM Team successfully produced again four active bacterial laccases:
All of the bacterial laccases were accomplished to purify. The purified laccases . Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol. The laccase of TVEL0 fromTrametes versicolor was produced and tested for activity on oaxidation of ABTS.
Subtrate Analysis
Since Amsterdam team activity test, team immobilization and team substrate analysis used laccases from the same pool. With these laccases we repeated the degradation experiments. For BPUL and ECOL more biological replicates were measured and the laccases BHAL and TTHL were characterized for estradiol and ethinyl estradiol degradation. Possible resulting degradation products were futher analysed via LCMS-MS.
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