In order to assess the feasibility using our Snifferomyces cells as a diagnosis method, a device called "Snifferometer" was built to measure the status of fluorescence. The purpose of building such a device is to overcome the complexity, incalculability, and the high cost of using fluorescence microscope.

Design of Snifferometer

Scheme of the Device

Figure 1: Scheme of Snifferometer

Figure 2: Real Snifferometer

Circuit of Snifferometer

Figure 3: Circuit of Snifferometer

Test

Eppendorf tubes are used to contain the liquid.

Two filters were tested:

• [http://www.leefilters.com/lighting/colour-details.html#768 LEE Filters 768 Egg yellow].
• [http://www.leefilters.com/lighting/colour-details.html#101 LEE Filters 101 yellow].

Four sets

• Control: M9 medium with E-coli
• Test: M9 medium with E-coli overexpressing TagGFP2

M9 medium is used because it has low autofluorescence.

Figure 4: Measured data

• reduce background light intensity: right filter
• increase green light intensity
• increase the sensitivity of the circuit
• enlarge the area of fluorescent