Team:HokkaidoU Japan/Notebook/aggregation Week 13

Characterization of Aggregation

Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT construct.

1. Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
2. Suspended colonies into the medium.
3. Incubated at 37C for 12 hrs at 180 rpm. For this incubation, pipetted 150 ul of this cultivating medium to 96-well microtiter plate every 2 hrs.
4. Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.

Result