Team:Paris Bettencourt/Overview

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1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth 2)Delay system: In presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not anymore present, the LacI repressor concentration goes down with dilution and degradation leading to the expression of the restriction enzyme.

1)Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth 2)Delay system: In presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not anymore present, the LacI repressor concentration goes down with dilution and degradation leading to the expression of the restriction enzyme. 3)Restriction Enzyme system: The restriction enzyme destroy a plasmid carrying an anti-toxin.

1)Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth 2)Delay system: In presence of Arabinose, LacI is produced repressing the expression of a restriction enzyme. Once Arabinose is not anymore present, the LacI repressor concentration goes down with dilution and degradation leading to the expression of the restriction enzyme. 3)Restriction Enzyme system: The restriction enzyme destroy a plasmid carrying an anti-toxin cassette 4)Suicide system: Once the anti-toxin is below a given threshold the toxin is not anymore titrated , the toxin kills the cell, its neighbors, and eliminates extracellular DNA via its DNase activity.

Semantic Containment.

1) Physical containment: Our cells will be encapsulated with Arabinose and LB in order to avoid release of DNA/cells and to permit growth

Objectives

Our project aims to:

• Raise the issue of biosafety, and advocate the discerning use of biosafety circuits in future iGEM projects as a requirement
• Evaluate the risk of HGT in different SynBio applications
• Develop a new, improved containment system to expand the range of environments where GEOs can be used safely.

To do so, we:

• Engaged the general public and scientific community through debate
• Raised the question about how we can regulate this practices
• Compiled a parts page of safety circuits in the registry
• Relied on three levels of containment :
  1. Physical containment with alginate capsules
  2. Semantic containment using an amber suppressor system
  3. An improved killswitch featuring delayed population-level suicide through complete genome degradation.

We strived to make our system as robust against mutations as possible.

Step 1

Step 2

Step 2.

Step 3

Step 3.