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Team:University College London/Module 2/Results
From 2012.igem.org
Module 2: Aggregation
Description | Design | Construction | Characterisation | Shear Device | Modelling | Results | Conclusions
Congo Red Agar Assay
We have used a congo red agar assay to determine if our curli BioBrick transformed cells have successfully expressed the amyloid fibrins. Congo red is a diazo dye that binds to the curli fibrins, thereby causing cells positive for curlis to appear red when grown on this agar.
As can be seen from plates below, our cells transformed with BBa_K729017 display a red tinge to their colonies, while the untransformed W3110 cells do not. We hence conclude that we have successfully transformed our cells to produce curlis.
| Control | BBa_K729017 |
|---|---|
 | 
|
Shear Force Assay
Using a shear device...
Based on the results we have obtained, we have determined that the biofilms produced by cells transformed with BBa_K729018 have a two-fold shear resistance as compared to the biofilms of the control W3110. This gives corroborating evidence that our curli BioBrick is functioning as intended, as curli generated biofilms are more resistant to shear forces, and detach less readily.
