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V08_06
V08_06_1 2nd round: Ethanol precipitation of mutated plasmids
- Experiment:
The ethanol precipitation was performed according to protocol.
V08_06_2 2nd round: Transformation of electrocompetent cells with the mutant plasmid mixture
- Experiment:
Electrocompetent cells were prepared according to protocol. The transformation was performed according to the established protocol.
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V08_07
V08_07_1 2nd round: Analysis of transforamtion V08_06
- Experiment:
Plates and liquid culture were investigated.
- Observations & Results:
Neither the plates nor the liquid culture exhibited bacterial growth. A test gel of the ethano precipitated ligation revealed that, again, our DNA material was lost during the process.
V08_07_2 2nd round: Mutagenesis PCR was set up again, 1000 µL
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V08_08
V08_08_1 2nd round: Analysis of transforamtion V08_06
- Experiment:
Plates and liquid culture were investigated.
- Observations & Results:
Neither the plates nor the liquid culture exhibited bacterial growth. A test gel of the ethano precipitated ligation revealed that, again, our DNA material was lost during the process.
V08_08_2 2nd round: Mutagenesis PCR was set up again, 1000 µL
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V08_09
V08_09_1 2nd round: Analysis of transforamtion V08_06
- Experiment:
Plates and liquid culture were investigated.
- Observations & Results:
Neither the plates nor the liquid culture exhibited bacterial growth. A test gel of the ethano precipitated ligation revealed that, again, our DNA material was lost during the process.
V08_09_2 2nd round: Mutagenesis PCR was set up again, 1000 µL
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V08_10
V08_10_1 2nd round: Analysis of transforamtion V08_06
- Experiment:
Plates and liquid culture were investigated.
- Observations & Results:
Neither the plates nor the liquid culture exhibited bacterial growth. A test gel of the ethano precipitated ligation revealed that, again, our DNA material was lost during the process.
V08_10_2 2nd round: Mutagenesis PCR was set up again, 1000 µL
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