Module 6: Containment

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

Conclusion

We have successfully transformed our cells to produce a periplasmic nuclease, a necessary feature to prevent transformation of wild-type cells in the marine environment. Furthermore, we have proven that this BioBrick does not adversely affect the viability of our cells, allowing it to be utilised as part of a containment system for engineered cells.

Future work would attempt to construct a three-fold containment system, in order to create a more robust system capable of prevent other mechanisms of horizontal gene transfer, such as transduction and bacterial conjugation.