Team:Goettingen/week14-3

Revision as of 13:54, 26 September 2012 by Alicia (Talk | contribs)


Home Homepage News Team Members Focus Groups Seminars Work Impressions Project Our Project Results Discussion/Outlook Lab Work Flow Notebook Overview Materials Methods Computational Data Bioinformatical Tools iGEM What is iGEM? Parts Submitted Attributions Safety > Göttingen City University of Göttingen Max-Planck-Institute S1-Demo Lab Human Practice Our Human Practice Projects Public and Media Survey Synthetic Biology Day Panel Discussion Flash Coli Sponsoring Our Sponsors Interested in...?
Deutsch / English

#3 Chemoreceptor Library - 14th Week

V07_30_1 2^nd round: Analysis of the transformation V07_27

Observations & Results:
Neither the liquid culture nor the plates exhibited bacterial growth.

V07_30_2 2^nd round: Saturated mutagenesis PCR, 1000 µL

Experiment:
The PCR was set up again, seeing as we lost our DNA material during the ethanol precipitation the previous week.

V07_31_1 2^nd round: PCR clean-up

Experiment:
The PCR clean-up was performed according to the established protocol form week 10.

Observations & Results:
The corresponding agarose gel showed a band of the expected size.

V07_31_2 2^nd round: DpnI/BsaI digestion and purification

Experiment:
The digestion was performed with a total volume of 300 µL according to protocol of week 10. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!

Observations & Results:
The corresponding agarose gel showed a band of the expected size.

V07_31_3 1^st round: Ligation

Experiment:
The ligation was set up in a 100 µL batch according to protocol. Incubation over night at 16 °C.

V08_01_1 2^nd round: Ethanol precipitation if ligation V07_31

Experiment:
The ethanol precipitation was performed according to the established protocol form week 10.

Observations & Results:
The corresponding agarose gel showed a band of the expected size.

V08_01_2 2^nd round: Transformation of electrocompetent cells with mutant plasmid mixture

Experiment:
Electrocompetent cells were prepared according to protocol. The transformation was performed according to protocol of week 10.