Team:BostonU/Notebook

• Our Team
  • Team
  • Summer Fun
• Project
  • Project Overview and Abstract
  • Introduction to Characterization
  • Methodology Overview
  • Results Summary
• Achievements
  • Data Collected
  • Parts Submitted
  • MoClo Kit
  • Medal Fulfillment
• Notebook
  • Protocols
  • Clotho and Eugene
  • Weekly Notebook
• Considerations
  • Synthetic Biology Advancement
  • Human Practices
  • Safety
• Acknowledgements
  • Collaborations
  • Acknowledgements

Notebook

May
Week 5:

• Orientation day

We met with our mentors and got an overview of the basics of synthetic biology, namely characterization, which will be the focus of our works during the summer. We also went through the “MoClo” technique, which we intend to introduce to iGEM.

• We did a survey of previous iGEM teams that got the Best Experimental Measurements award, then presented the findings to our mentors.

June
Week 1

• We performed PCR to amplify pMJSAF using the following MoClo forward and reverse primers.
• DVL0_AF with DVL0_BR (MF1)
• DVL0_BF with DVL0_CR (MF2)
• DVL0_CF with DVL0_DR (SJ1)
• DVL0_DF with DVL0_ER (SJ2)

• We performed Gel Electrophoresis to confirm that pMJSAF was amplified in PCR.