Team:Goettingen/week14-3

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#3 Chemoreceptor Library - 14th Week

V07_30_1 2^nd round: Analysis of the transformation V07_27

Observations & Results:
Neither the liquid culture nor the plates exhibited bacterial growth.

V07_30_2 2^nd round: Saturated mutagenesis PCR, 1000 µL

Experiment:
The PCR was set up again, seeing as we lost our DNA material during the ethanol precipitation the previous week.

V07_31_1 2^nd round: PCR clean-up

Experiment:
The PCR clean-up was performed according to the established protocol form week 10.

Observations & Results:
The correspodning agarose gel showed a band of the expected size.

V07_31_2 2^nd round: DpnI/BsaI digestion and purification

Experiment:
The digestion was performed with a total volume of 500 µL according to protocol of week 10. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!

V07_31_3 1^st round: Ligation

Experiment:
The ligation was set up in a 100 µL batch according to protocol. Incubation over night at 16 °C.