Team:Paris Bettencourt/Restriction Enzyme

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Overview

Our group was responsible for designing self-plasmid digestion system. This synthetic system allows to digest plasmids into linear parts of DNA which afterwards could be degraded by Colicin.

Objectives

1. To find appropriate restriction enzymes which have to match the next properties:
   • In the E.Coli genome there is no restriction sites of a choosen restriction enzyme;
   • It has to have high specifity;
   • It have to works in wide range of different conditions (pH, T°C, etc)
2. Choose very strong promoter to regulate restriction enzyme expression;
3. To clone circuits with different combinations of choosen restriction enzymes and promoters.
4. Mesure degradation efficiency for each circuit.
5. Based on the best combinantion design self-disruption plasmid.

Design

Present the design of your system, both in a written form, and a schematic one.

Experiments and results

Characterisation of X

Experimental setup

Describe the experiment

Results

Present your results

Testing of the system

Experimental setup

Describe the experiment

Results

Present your results