Team:Goettingen/week13-3

Revision as of 15:01, 24 September 2012 by Alicia (Talk | contribs)


Home Homepage News Team Members Focus Groups Seminars Work Impressions Project Our Project Results Discussion/Outlook Lab Work Flow Notebook Overview Materials Methods Computational Data Bioinformatical Tools iGEM What is iGEM? Parts Submitted Attributions Safety > Göttingen City University of Göttingen Max-Planck-Institute S1-Demo Lab Human Practice Our Human Practice Projects Public and Media Survey Synthetic Biology Day Panel Discussion Flash Coli Sponsoring Our Sponsors Interested in...?
Deutsch / English

#3 Chemoreceptor Library - 13th Week

2^nd round: Analysis of plates and liquid culture V07_20

Experiment:
Both the liquid culture and the plates exhibited bacterial growth. 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.

V07_25_1 2^nd round: Plasmid preparation clones 1-14 for subsequent sequencing

Experiment:
Plasmid preparation was performd with peqGOLD Plasmid Miniprep Kit (PeqLab) following the user manual. DNA concentrations were determined via nanodrop for subsequent sequencing.

V07_25_2 2^nd round:

2^nd round: