Team:Goettingen/week3-3

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#3 Chemoreceptor Library - 3rd Week

Preparing Media

Experiment:
Preparation of LB medium and LB-Amp plates (recipe see here). Preparing streak plates of pUC18 DH10B on LB-Amp, incubation at 37 °C.

Isolation of pUC18 plasmid DNA

Insertion of tar into pUC18

Experiment:
Double digest of tar and pUC18 using EcoRI, PstI and Orange Buffer (ThermoScientific). Preparation of chemical competent DH10B cells for transformation.