Team:Goettingen/week3-2

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#2 Speed Improvement - 3rd week

Preparation of overnight cultures

Experiment:
Over night cultures for the preparation of chemically competent cells were prepared using E. coli (DH10B) cells.

V05_18_1 Double Digestion of flhDC and pUC18

Experiment:
Both parts were digested with EcoRI and PstI using Orange Buffer.

V05_18_2 Preparing chemically competent cells of E.coli (DH10B)

Experiment:
The preparation of the competent cells was performed according to the following protocol.