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#2 Speed Improvement - 2nd week Back to overview V05_08 Amplification of flhDC gene Experiment: Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol. V05_10 Separation of flhDC Experiment: The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used. Observations & Results: The gel featured a clear band at about 900 kb. The amplification of flhDC was successful. Back to overview V05_11 Purificaton of flhDC Experiment: The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.

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