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Team:HokkaidoU Japan/Notebook/plastic Week 11
From 2012.igem.org
Contents |
September 10th
PCR
PhaA was multiplied from pGEM with XbaI and SpeI restriction sites by PCR.
Digestion
PhaA was digested with XbaI and SpeI. RBS on pSB1A2 was digested with SpeI.
Gel extraction
We confirmed succession of digestion by electrophoresis.
And then DNA were extracted from TBE gel.
ligation
RBS on pSB1A2 was ligated with PhaA and PhaB.
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