Team:University College London/Protocols/RestrictionEnzymeDigest1

Revision as of 15:35, 16 July 2012 by PhilippBoeing (Talk | contribs)

== Enzyme Digest ==

1. Thaw all materials on ice

2. Add the following ingredients to autoclaved/sterile eppendorf tubes

Insert Table

3.Flick contents gently and centrifuge.

4.To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight

5. Then run on the following conditions (by turning the dial)

RPM: 14000

Time: 1 minute

Temperature: 18oC