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Team:BostonU/DataSheet
From 2012.igem.org
Data Sheet
In other fields of engineering, such as electrical engineering, data sheets are a way to document part data such that the behavior of the part is captured and can be shared with other groups. Ideally, the information on these sheets are robust enough to allow any group to use the part in a different context effectively, given the information captured on these data sheets.
Canton et al. 2008 Arkin 2008
Several groups have started developing the ideas that should be on such a data sheet for DNA parts. Canton et. al included information such as performance, reliability and compatibility. Arkin suggested that general device data, cell-cell communication measurements, DNA-binding protein domain measurements and therapeutic measurements could be made in also. A sharing standard is sought, but there is still no standard mechanism for sharing part information amongst synthetic biologists.
Our approach to sharing characterization data through a data sheet is to break information into four major sections: General Datasheet Information, Growth/Measurement Conditions, Part Information and Data Analysis.
General Datasheet Information:Here a brief abstract of the findings of the characterization, author and date information are included in addition to other relevant comments and conclusions. Also included here would be links or references to related parts and datasheets.
Growth/Measurement Conditions: In this section, all experimental growth conditions and measurement settings are documented. The experimental conditions include controls used and is similar to an abbreviated methods section in a journal article. This would include who built the parts and what assembly method/protocol are used. It also includes when during the process things are tested and how. Some popular measurement techniques are based on fluorescence of a molecular fluorophore such as microscopy, flow data, or plate reading.
· Device name
· Part names (no sequence data)
· Assembly confirmation – gel (image?) and seq info (not the full seq, just if it has been seq’ed and if it’s correct; text, no image)
· Figure legends for all images used
· Pieces are only for human consumption, but we’re working on making them consumable with a computer program
[1] B. Canton et al. “Refinement and standardization...” Nature Biotech 26(7). 2008.
[2] A. Arkin "Setting the standard in synthetic biology" Nature Biotech 26(7). 2008.
