Team:BostonU/Data
From 2012.igem.org
Data Collected
It is unclear if the σ54 promoter will function in E. coli in the absence of mmoR. nBLAST results comparing the mmoR to the E. coli genomes available yielded very short hits, with the top hit showing matches for less than 200bp out of the possible 2070bp length of mmoR. However, the pBLAST hits showed stronger hits with other σ54-dependent transcriptional activators found in E. coli. The top nBLAST and pBLAST hits are shown below.
To determine if the σ54 promoter will function without mmoR present in E. coli, we placed the promoter in front of GFP, RFP, or YFP.