Module 4: Buoyancy

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

Characterisation of Starvation Promoter BBa_K118011

Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.

We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.