Team:TMU-Tokyo/Notebook/Experiment/5th week (9.10 - 9.16)

■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)

Experiment

■5th week (9.10 - 9.16)

■10th Sep

STEP 3: Construction of Device2
PCR of insert (FDH)
Electrophoresis
Failure: because of a lot of smear
Ordered Device1 and Device2 plasmids arrive!


■11th Sep

STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of no bands
■12th Sep


STEP 1: Construction of Device3
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss
Electrophoresis
Failure: because of transformation miss


■13th Sep


STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Success: We observed appropriate bands but a lot of smear
Colony PCR of transformed E. coli (p-fdh4AB)
Electrophoresis
Failure: because of no appropriate bands


■14th Sep
New fdh4AB primers arrive
STEP 1: Construction of Device3
PCR of insert (fdh4AB)
Electrophoresis
Failure: because of artificial mistake?
PCR of insert (fdh4AB)
Electrophoresis
Success: appropriate bands are clearer than before!