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From 2012.igem.org

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Contents 1 Computational Data 1.1 Maps 1.1.1 Tar_QC 1.1.2 flhDC 1.1.3 fliC 1.1.4 motA 1.1.5 motB 1.1.6 yhjH 1.1.7 Constitutive promoter + RFP-constructs 1.2 Sequences 1.2.1 Primers

Computational Data

Maps

Tar_QC

For Parts K777000 - K777008 the plasmid maps were constructed in ApE. Within the maps you will find annotated features, as well. To gain more information about the parts submitted by the iGEM team Göttingen 2012 click here.
Moreover, the parts were completely sequenced (except for K777000) and aligned. The original data and the reviewed data are provided in the respective Zip- files.

• ApE plasmid maps
• Sequencing results
  • K777000 in pSB1C3
  • K777001 in pSB1C3
  • K777002 in pSB1C3
  • K777003 in pSB1C3
  • K777004 in pSB1C3
  • K777005 in pSB1C3
  • K777006 in pSB1C3
  • K777007 in pSB1C3
  • K777008 in pSB1C3

flhDC

• K777100 in pSB1C3 ApE plasmid map

fliC

• K777109 in pSB1C3 ApE plasmid map

motA

• K777113 in pSB1C3 ApE plasmid map

motB

• K777117 in pSB1C3 ApE plasmid map

yhjH

• K777121 in pSB1C3 ApE plasmid map

Constitutive promoter + RFP-constructs

• K777125-K777132 ApE plasmid maps

Sequences

Primers

• Mutagenesis
• PCR
• QuikChange
• Sequencing

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