Labjournal

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Week 10 (07/02 - 07/08/12)

Monday July 2nd

• Sending a request for plasmids containing cDNA sequences of five different laccases from Trametes versicolor and Pycnoporus cinnabarinus from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald.

Tuesday July 3rd

Wednesday July 4th

• Team Student Academy: Final meeting with all instructors of the student academy to talk about all details.

Thursday July 5th

• Team shuttle vector: Some fragments for Gibson Assembly with overlapping DNA sequences were amplified via Phusion-PCR. The fragment 5'AOX1 was amplified with the primerpair pSB1C3-5aox1-f and pSB1C3-5aox1-r. The fragment MF-alpha1 was amplified with the primerpair 5aox1-mfalpha1-f and 5aox1-mfalpha1-r. The fragment 3'AOX1 was amplified with the primerpair his4-3aox1-f and his4-3aox1-r. The fragment pSB1C3 was amplified with the primerpair 3aox1-pSB1C3-f and 3aox1-pSB1C3-r.

Friday July 6th

• Team Student Academy: The two presentations were prepared and the barbecue for the pupils was organized. In the lab the last preparations were made.

Saturday July 7th

Sunday July 8th