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Team:Bielefeld-Germany/Labjournal/week3
From 2012.igem.org
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18 Week19 Week20 Week21
Week 3 (05/14 - 05/20/12)
weekly seminar:
- first lab service: Robert
- our GFP, which we wanted to use for the summer school for pupils, does not work
- first competent cells have to be made: Julia S. and Robert
- Decision to buy a commercial laccase to establish the analytics and the enzyme tests
- our expose has to be translated into english: Malak
- last planning for our waver sell
- Julia S. is creating a vector for Pichia pastoris and is now looking for sequences
- The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process
Monday May 14th
Tuesday May 15th
- Team Activity Tests: For some pre test and characterization for our future laccase activity standard we ordered [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de®ion=DE laccase] from Trametes versicolor. As well we had to order a substrate that the laccase could use to demonstrate its abilities. According to the literature [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de®ion=DE ABTS] is a well working substrate to characterize oxidizing enzym activity. So we ordered.
Wednesday May 16th
- Team Bacterial Laccases: For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.
Thursday May 17th
- Team Modeling: Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
