Team:NTU-Taida/PEPDEX

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Project

Testing Hero

Contents

Overall project

In our project, we aim to utilize a microbe that responds to conditions in human body as an approach to administer smart peptide-based therapies. GLP-1, a human innate neuro-peptide for energy balance, is chosen to combat for obesity and metabolic syndrome. We engineer the non-pathogenic E. coli which senses fatty acids in intestines and secretes synthetic GLP-1. Appropriate signal peptides and penetratin are used to facilitate peptide secretion and intestinal uptake. Furthermore, we design a circuit with quorum sensing and double repressors, which aims to generate quick but sustainable responses and serves as an anti-noise filter. Plasmid stabilization modules including partition system and multimer resolution system are also incorporated to circumvent the undesirable loss or segregational instability of our artificial device. With this general concept of delivery of short peptide into human body, we can also target other human diseases with alternative circuit designs.

Project Details

hormone therapy-body weight control

Background

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Design

CPP File:Https://www.dropbox.com/s/1lnt1d2uo401rz6/123.mov fadR


FadR-DNA.png


Modeling

Results

Extension

peptide2

Background

Design

Modeling

Results

Extension

delivery circuit

Background

Design

Modeling

Results

Extension

Stability of delivery system

Briefing

As our system will function outside the labrotory and human gut lack for antibiotic selection pressure, the vector stability is the critical point that determine whether our system is applicable or not. Inspired by natural plasmid & transposable gene element, we cope up with vector instability by incorporating partition system, Multimer resolution system and toxin antitoxin system these modules into our design.

Background

plasmid instability

multimer resolution system
A description list is perfect for defining terms.
partition system
A description list is perfect for defining terms.
Post-segregation killing
A description list is perfect for defining terms.

Design

multimer resolution system:Tn1000(gamma delta) resolution system

NTU-Taida-Mrs-1.png NTU-Taida-Mrs-2.png NTU-Taida-Mrs-3.png NTU-Taida-Mrs-4.png

partition system:from pseudomonas putida KT2440

Post-segregation killing:srnBC toxin-antitoxin system


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reference

  • 1.reference one
  • 2.reference two

safety of delivery system

suicidal system:

     Level 1 of column
Level 2
Level 2

Horizontal gene transfer resister!!!

Modeling

Results

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Extension