Team:TMU-Tokyo

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Project Abstract

Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1) We planed to create E.coli with an ability to　detect and detoxify formaldehyde., named Chef Ant E.coli. About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP. We plan to overexpress　two enzymes in E.coli. First,formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida. Second,formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methylobacterium extorquens.