Team:HokkaidoU Japan/Notebook/plastic Week 8

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Contents

August 20th

A digestion to divide PhaC and pSB1C3 using .

DNA solution PhaC ( 257ng/ul) 12 ul
XbaI 1 ul
SpeI 1 ul
10xM buffer 2 ul
DW 4 ul
Total 20 ul


August 21th

Colony PCR

August 22th

Colony PCR


August 23th

Colony PCR

August 24th

Colony PCR


August 25th

Colony PCR

August 26th

Mini prep of DNA