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Team:Bielefeld-Germany/Labjournal
From 2012.igem.org
Contents |
Prologue
Starting the Team
Beginning in january and february members of the former iGEM team from Bielefeld started seminars to inform interested students about synthetic biology, iGEM and the past Bielefeld projects. In March the final 2012 iGEM Bielefeld team was formed of 15 students and weekly meetings began. First project ideas were:
- the detection of multiresistent pathogens
- communication between bacteria ang funghi using quorum sensing
- a bacterial hand warmer
- a possibility to detect and destroy mold fungus
- something about spontaneous combustion of hay bale
- an enzyme dispenser
Labjournal
Week 1 (30.04.-06.05.12)
- Start of our WET LAB time.
- Generating new competent E.coli cells.
- Cultiviation of Xanthomonas campestris B100 and E. coli BL21, which we got from a working group at our University, and isolation of genomic DNA. The genomic DNA was needed as template for PCRs to purify the wanted laccase ORFs.
- weekly seminar:
- Do we want to order strains of Trametes versicolor and Trametes villosa?
- Gathering information about signal sequences in yeast
- Decision to create a database, so that we can easily number and inscribe our lab results
- Decision to arrange a summer school for pupils in their last year before the final exams
- Discussion about how to meet a member of the german Bundestag (the german parliament)
Week 2 (07.05.- 13.05.12)
- Doing the first steps to prepare a the Student Acadamy in cooperation with the CeBiTec. Finding suitable BioBricks to develop an coulourful and easy experiment. Trying to isolate the GFP and RFP containing plasmid and to transform an Plasmidmix to produce colored Agar-plates.
- Primerdesign for isolation of laccases from genomic DNA of Xanthomonas campestris B100, E. coli BL21(DE3) and Bacillus pumilus ATCC7061. The primers were designed with T7 promotor-overhanging ends after prefix and HIS-Tag before suffix.
- Successful PCRs of laccase genes CopA from Xanthomonas campestris B100, CueO from E. coli BL21(DE3) with the isoltated genomic DNA as template.
- Successful PCR of laccase gene CotA from Bacillus pumilus ATCC7061. As template we got a vector with the laccase-ORF from the Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials in Switzerland.
Week 3 (14.05.- 20.05.12)
- Cloning of CueO (E. coli), CopA (Xanthomonas campestris) and CotA (Bacillus pumilus) in pSB1C3-backbone.
Week 4 (21.05.- 27.05.12)
- Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.
Week 5 (28.05.- 03.06.12)
- Cloning of CotA (Bacillus halodurans), Tth-laccase (Thermus thermophilus) and ccdB in pSB1C3-backbone.
