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Team:Valencia Biocampus/Notebook
From 2012.igem.org
Notebook
12th July: Today, the bacterial cultures made the previous day grew succesfully, the [bacteria sub-team] components stringed several colonies and grew them in LB+Amp liquid media.
At lunchtime the [bacteria sub-team] and the [yeast sub-team] had a nice meeting to discuss some issues related to the project.
11th July: In this amazing day, the [bacteria sub-team] performed some bacterial cultures, more specifically they plated using triple groove in order to get isolated colonies which ones wolud be stinged and grown in a selective media (37ºC, 200 rpm in a stirring chamber) the next day. Moreover, the [bacteria sub-team] made new media: LB and LB-agar.
10th July: In this sunny day, the [bacteria sub-team] and the [yeast sub-team] have made an electrophoresis using the DNA which has been digested with EcoRV over-night. The results obtained were uneseful because, like the sub-team components discovered, the enzyme EcoRV was used to introduce the insert into the vector pUC57 so the cleavage site wasn't present anymore (the enzyme EcoRV cuts resulting in blunt ends).
9th July: Today, the [bacteria sub-team] and the [yeast sub-team] have made several mini-preps in order to purify the DNA constructions. The next step has been to test if the mini-preps have been successful. To do it, the sub-teams have digested these constructions (using restriction enzymes: EcoRI, PstI) and they have made an agarose gel in order to do an electrophoresis with the digested DNA. After that, they have revealed the gel and they have found out that the results were unexpected, so they have decided to make another digestion, in this case over-night and using only EcoRI, and carry out the subsequent electrophoresis tomorrow.
Moreover, the [poster sub-team] have made a brain-storming to start designing the logo for the team and the have drawn the first sketches.
8th July: Today, the [bacteria sub-team] and the [yeast sub-team] have transfered our transformed E.coli colonies to liquid media to make them grow over-night to manipulate them tomorrow.
6th July: Today, the [bacteria sub-team] and the [yeast sub-team] have transformed E.coli with the different constructions using the [Transformation Protocol Using Heat Shock]and have plated them in LB+Amp plates.
5th July: Today, the [bacteria sub-team] has finished all the culture media (for each construction). The bacteria sub-team has had lunch with the yeast sub-team and we have discussed some aspects about the project. Also, [yeast sub-team] has made the experimental protocol for next week.
4th July: Today, the [bacteria sub-team] has started to make up their bacteria media (L.B. and defined media).
3th July: Today, the [modelling sub-team] has made a meeting in order to share the deduced equations and decide the definitive ones. Later, it has been decided to search information which could be useful to characterize the behavior of E. coli systems and S. cerevisiae system
21th June: Today, the [modelling sub-team] has made a meeting where the advisors have explained the bases of modelling for synthetic biology. After that, the students have been responsible of deducing the equations that characterize our project.
